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Assessment of Anti-inflammatory Effects of Main Components of ESS in RAW 264.7 Macrophages. Chemical structures of the major components of ESS were created ( A ) Sargaquinoic acid, ( C ) Sargahydroquinoic acid, and ( E ) Sargachromanol using <t>Chemsketch</t> software. RAW 264.7 cells were pre-treated with ( B ) SQA, ( D ) SHQA, and ( F ) SCM at concentrations of 2.5 and 5 μg/ml, respectively, for 1 h and then exposed to LPS for 24 h. Cytotoxicity was assessed using the MTS assay, and anti-inflammatory activity was determined by measuring NO production. The data is presented as mean ± SEM and statistical analysis was conducted for each gene. Significant differences are denoted as follows: # p < 0.005 and ### p < 0.001 when compared to the untreated group, and * p < 0.005, ** p < 0.01, *** p < 0.001 when compared to the LPS-treated group.
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Assessment of Anti-inflammatory Effects of Main Components of ESS in RAW 264.7 Macrophages. Chemical structures of the major components of ESS were created ( A ) Sargaquinoic acid, ( C ) Sargahydroquinoic acid, and ( E ) Sargachromanol using <t>Chemsketch</t> software. RAW 264.7 cells were pre-treated with ( B ) SQA, ( D ) SHQA, and ( F ) SCM at concentrations of 2.5 and 5 μg/ml, respectively, for 1 h and then exposed to LPS for 24 h. Cytotoxicity was assessed using the MTS assay, and anti-inflammatory activity was determined by measuring NO production. The data is presented as mean ± SEM and statistical analysis was conducted for each gene. Significant differences are denoted as follows: # p < 0.005 and ### p < 0.001 when compared to the untreated group, and * p < 0.005, ** p < 0.01, *** p < 0.001 when compared to the LPS-treated group.
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Assessment of Anti-inflammatory Effects of Main Components of ESS in RAW 264.7 Macrophages. Chemical structures of the major components of ESS were created ( A ) Sargaquinoic acid, ( C ) Sargahydroquinoic acid, and ( E ) Sargachromanol using Chemsketch software. RAW 264.7 cells were pre-treated with ( B ) SQA, ( D ) SHQA, and ( F ) SCM at concentrations of 2.5 and 5 μg/ml, respectively, for 1 h and then exposed to LPS for 24 h. Cytotoxicity was assessed using the MTS assay, and anti-inflammatory activity was determined by measuring NO production. The data is presented as mean ± SEM and statistical analysis was conducted for each gene. Significant differences are denoted as follows: # p < 0.005 and ### p < 0.001 when compared to the untreated group, and * p < 0.005, ** p < 0.01, *** p < 0.001 when compared to the LPS-treated group.

Journal: Scientific Reports

Article Title: Modulation of macrophage transcript and secretion profiles by Sargassum Serratifolium extract is associated with the suppression of muscle atrophy

doi: 10.1038/s41598-024-63146-0

Figure Lengend Snippet: Assessment of Anti-inflammatory Effects of Main Components of ESS in RAW 264.7 Macrophages. Chemical structures of the major components of ESS were created ( A ) Sargaquinoic acid, ( C ) Sargahydroquinoic acid, and ( E ) Sargachromanol using Chemsketch software. RAW 264.7 cells were pre-treated with ( B ) SQA, ( D ) SHQA, and ( F ) SCM at concentrations of 2.5 and 5 μg/ml, respectively, for 1 h and then exposed to LPS for 24 h. Cytotoxicity was assessed using the MTS assay, and anti-inflammatory activity was determined by measuring NO production. The data is presented as mean ± SEM and statistical analysis was conducted for each gene. Significant differences are denoted as follows: # p < 0.005 and ### p < 0.001 when compared to the untreated group, and * p < 0.005, ** p < 0.01, *** p < 0.001 when compared to the LPS-treated group.

Article Snippet: The chemical structures of all these molecules were drawn by using ACD/ChemSketch software (version 2023, https://www.acdlabs.com/resources/free-chemistry-software-apps/chemsketch-freeware/ ).

Techniques: Software, MTS Assay, Activity Assay